Al-mayyahi: Neurosphere

Cryostat section of a neurosphere

Cryostat section of a neurosphere.

Cryostat section of a neurosphere: neural stem cells are isolated from the subventricular zone of mouse brain and cultured in neurosphere medium in presence of growth factors and hormonal supplement to generate non- adherent spherical clusters of undifferentiated cells called neurospheres. Nestin (red), a marker protein for neural stem cells, EdU (green), was used to label proliferating cells and DAPI (blue) was used as a nuclear stain. From the work of Rawaa Al-mayyahi, Chari Group, Keele University.